MRI Signaling Method Designed to Visualize Disease Metabolism in Action

U.S. chemists are utilizing modified magnetic resonance imaging (MRI) to see molecular changes inside people's bodies that could signal health problems such as cancer.

Their new method, reported in the March 27, 2009, issue of the research journal Science, makes more of the body's chemistry visible by magnetic resonance imaging (MRI), according to Dr. Warren S. Warren, professor of chemistry at Duke University (Durham, NC, USA).

Conventional MRI and functional MRI (fMRI) used for brain imaging enlist the hydrogen atoms in water to create a graphic display in response to magnetic pulses and radio waves. But a huge array of water molecules is needed to pull that off. "Only one out of every 100,000 water molecules in the body will actually contribute any useful signal to build that image," Dr. Warren said. "The water signal is not much different between tumors and normal tissue, but the other internal chemistry is different. So detecting other molecules, and how they change, would aid diagnosis."

The Duke team has been able to see these other molecules with MRI by "hyperpolarizing" various atoms in a sample, adjusting the spins of their nuclei to significantly increase their signal. This creates large imbalances among the populations of those spin states, making the molecules into more powerful magnets.

Unlike standard MRI, hyperpolarization and a technique called dynamic nuclear polarization (DNP), which was used for this research, can produce strong MRI signals from a variety of other kinds of atoms besides water. Without hyperpolarization, detecting signals from atoms besides water is extremely difficult because the signal size is so small. But "these signals are strong enough to see, even though the molecules are much more complex than water," Dr. Warren stated.

Dr. Warren's group uses what he calls the "first DNP hyperpolarizer in the South," which is installed in his laboratory. It also uses Duke's Small Molecule Synthesis Facility to create custom molecular architectures. "You thus have a signal that, at least transiently, can be thousands or ten thousands times stronger than regular hydrogen in an MRI," Dr. Warren said. "It lets you turn molecules you are interested in into MRI light bulbs."

Duke's hyperpolarizer includes a superconducting magnet, a cryogenic cooling system that initially plunges temperatures to a scant 1.4 Kelvin while microwave radiation transfers spin polarization from electrons to nuclei, and a heating system to rapidly warm the molecules back up.

Hyperpolarized spin states do not last for long inside the body, but ways have been found to lengthen them. Several years ago, another group of researchers discovered a method to make DNP work at room temperature in some biologic molecules by substituting carbon-13 atoms for some of those molecules' normal carbon-12s. Unlike carbon-12, carbon-13 emits an NMR signal like hydrogen atoms do.

Using this room temperature DNP, the biologic molecule pyruvate can retain its MRI signal for as long as 40 seconds--long enough to observe it undergoing rapid chemical change. "So you can watch pyruvate metabolize to produce lactate, acetic acid, and bicarbonate--all breakdown products that might correlate with cancer," Dr. Warren said. But most biologic processes are much slower, and thus cannot be seen with this method.

In the study, the Duke team described a new method that can additionally extend the signals of molecules carrying exchanged carbon-13s. It works by temporarily bottling-up the hyperpolarization in the longest-lived spin states--called singlet eigenstates--within specially designed molecular architectures. "You can actually use their own chemistries to get the molecules in and out of those protected states," Dr. Warren commented.

For example, hyperpolarized populations locked within a specially prepared form of diacetyl, a bacterially made chemical that imparts buttery flavoring to foods, can be stored using this method, according to the report. Once triggered, the MRI signal can be extended over many minutes before the spin states decay.

Thus bottled-up, the signals could be kept in temporary isolation. By dehydrating and shielding them from water within microscopic capsules, for example, these "signaling" molecules could be transported through the bloodstream to a potential disease site, according to Dr. Warren. Once at that site, a focused burst of ultrasound or heat could restore the molecules' missing water. That would cause a telltale signal to be released just as a rapidly progressing metabolic event was unfolding.

The Duke group is evaluating the potentials for a number of other possible signaling molecules, such as those involved in Parkinson's disease, bladder control, and osteoporosis, noted Dr. Warren, who has filed for a provisional patent.

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